Human Pentraxin 3/PTX3 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.99 pg/ml (50 μl)；9.53 pg/ml (10 μl) |
| Standard Curve Range | 13.73~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0431 | 0.0393 | 0.0412 | |
| 13.72 | 0.0704 | 0.0717 | 0.0711 | 0.0299 |
| 41.15 | 0.1370 | 0.1360 | 0.1365 | 0.0953 |
| 123.46 | 0.3247 | 0.3126 | 0.3187 | 0.2775 |
| 370.37 | 0.8276 | 0.7558 | 0.7917 | 0.7505 |
| 1111.11 | 1.7600 | 1.6140 | 1.6870 | 1.6458 |
| 3333.33 | 3.0380 | 2.8520 | 2.9450 | 2.9038 |
| 10000.00 | 3.8390 | 3.6580 | 3.7485 | 3.7073 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 213.5 | 1031.5 | 3290.1 | 242.9 | 1110.6 | 3753.9 |
| Standard Deviation | 11.3 | 63.9 | 223.3 | 5.3 | 51.7 | 224.3 |
| Coefficient of Variation(%) | 5.3 | 6.2 | 6.8 | 2.2 | 4.7 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Pentraxin 3/PTX3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1308.85-6892.74 | 100 | 3254.3 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Pentraxin 3/PTX3 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Pentraxin 3/PTX3
Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.
