Human Nectin-2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.80 pg/ml (50 μl)；3.86 pg/ml (10 μl)； |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0301 | 0.0336 | 0.0319 | |
| 6.86 | 0.0491 | 0.0494 | 0.0493 | 0.0174 |
| 20.58 | 0.0780 | 0.0772 | 0.0776 | 0.0458 |
| 61.73 | 0.1560 | 0.1675 | 0.1618 | 0.1299 |
| 185.19 | 0.4141 | 0.4033 | 0.4087 | 0.3769 |
| 555.56 | 1.0870 | 1.0920 | 1.0895 | 1.0577 |
| 1666.67 | 2.4910 | 2.4590 | 2.4750 | 2.4432 |
| 5000.00 | 4.1673 | 4.2113 | 4.1893 | 4.1575 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 123.6 | 608.0 | 1941.0 | 122.5 | 568.7 | 1788.8 |
| Standard Deviation | 5.3 | 34.8 | 149.9 | 3.3 | 17.4 | 38.6 |
| Coefficient of Variation(%) | 4.3 | 5.7 | 7.7 | 2.7 | 3.1 | 2.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Nectin-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 121% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 2.64-87.89 | 100 | 12.19 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Nectin-2 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Nectin-2
Nectins are type I transmembrane glycoproteins that are calcium-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs). Homology has led to their designation as poliovirus receptor-related (PRR) proteins, but nectins do not bind poliovirus. The extracellular domains of the nectin family form cis-homodimers (same cell), followed by homophilic or heterophilic trans-dimers (across cells) that organize intercellular junctions. Nectin-1, also known as CD111, PRR1 and herpes virus entry mediator C (HVEC) has a secreted splice variant. Nectin-1, Nectin-2 (CD112, PRR2, HVEB) and Nectin-3 (PRR3) are concentrated in adherens junctions and exist on neurons, endothelial cells, epithelial cells and fibroblasts. Nectin-4 is mainly expressed in the placenta; a soluble form is generated via the action of TACE/ADAM17.
