Human/Mouse IL-20 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.25 pg/ml (10 μl) |
| Standard Curve Range | 7.81~500 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0434 | 0.0467 | 0.0451 | |
| 7.81 | 0.1143 | 0.1205 | 0.1174 | 0.0724 |
| 15.63 | 0.1864 | 0.1869 | 0.1867 | 0.1416 |
| 31.25 | 0.3266 | 0.3311 | 0.3289 | 0.2838 |
| 62.50 | 0.5885 | 0.5804 | 0.5845 | 0.5394 |
| 125.00 | 1.0450 | 1.0350 | 1.0400 | 0.9950 |
| 250.00 | 1.7580 | 1.7400 | 1.7490 | 1.7040 |
| 500.00 | 2.9120 | 2.9250 | 2.9185 | 2.8735 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 26.4 | 121.0 | 357.4 | 25.4 | 120.0 | 348.5 |
| Standard Deviation | 0.8 | 4.4 | 16.4 | 1.1 | 4.0 | 6.6 |
| Coefficient of Variation(%) | 3.1 | 3.6 | 4.6 | 4.5 | 3.3 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/mouse IL-20 into health human/mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human serum | 30 | n.d. | 0 | n.d. |
| Mouse serum | 30 | 7.43-60.30 | 100 | 23.29 |
Serum/Plasma – Thirty samples from apparently healthy volunteers and thirty samples from apparently healthy mice were evaluated for the presence of human/mouse IL-20 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-20
The class II cytokine receptor family includes 12 members, 11 of which combine as various heterodimers to transduce signals across the cellular membrane for 27 cytokines. These cytokines are divided into four structurally related groups: six cytokines of the IL-10 family, 17 type I IFNs, one type II IFN and three IFN-lamda. IL-20 is a member of the IL-10 family of cytokines that has been shown to be upregulated in psoriatic skin. The receptor for IL-20 (IL-20R) exists as a dimer consisting of IL-20Ralpha and IL-20Rbeta. IL-20R also functions as a receptor for IL-19, IL-24 and IL-26. It is highly expressed in skin, brain and testis. IL-20, IL-20Ralpha and IL-20Rbeta all have higher expression levels in psoriatic lesional (LS) skin than in nonlesional (NL) skin, suggesting a role for these proteins as potential therapeutic targets. IL-20 can induce signal transducer and activator of transcription (Stat) activation through IL-20R.
