Human/Mouse GLP-1 Super Sensitivity enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 40.14 pg/ml (50 μl) |
| Standard Curve Range | 93.31~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl |
| Type | Ready-to-Use |
| Operation Duration | 120 min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0205 | 0.0206 | 0.0206 | |
| 93.31 | 0.0335 | 0.0346 | 0.0341 | 0.0135 |
| 155.52 | 0.0585 | 0.0593 | 0.0589 | 0.0384 |
| 259.20 | 0.1213 | 0.1202 | 0.1208 | 0.1002 |
| 432.00 | 0.2877 | 0.2675 | 0.2776 | 0.2571 |
| 720.00 | 0.6812 | 0.6207 | 0.6510 | 0.6304 |
| 1200.00 | 1.6720 | 1.5480 | 1.6100 | 1.5895 |
| 2000.00 | 3.4750 | 3.2990 | 3.3870 | 3.3665 |
Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human/mouse GLP-1 into health human/mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 111% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | n.d.-322.28 | 81.3 | 187.50 |
| Mouse Serum | 30 | 193.63-837.38 | 100.0 | 420.28 |
Serum/Plasma– Thirty samples from apparently healthy volunteers/mouse serum were evaluated for the presence of human/mouse GLP-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: GLP-1
Glucagon is a preprotein which is cleaved into four distinct peptides, including the hormone GLP-1 (aa 98-128). GLP-1 is a secreted hormone with multiple effects upon the intestine (gastric motility), pancreas (glucose dependent insulin release) and hypothalamic pituitary axis (modulates LH, THS, CRH, ocytocin and vasopressin secretion). It also affects plasma glucagon levels. GLP-1 is expressed in enteroendocrine L cells and neurons of the caudal brainstem which project to the forbain, the amygdala and the hypothalamus. Recent studies show pancreatic intra-islet GLP-1 expression, which is regulated by cytokines, hyperglycemia and cell injury.
