Human MMP-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 11.29 pg/ml (50 μl)；14.24 pg/ml (10 μl)； |
| Standard Curve Range | 40.96~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0274 | 0.0274 | 0.0274 | |
| 40.96 | 0.0411 | 0.0406 | 0.0409 | 0.0135 |
| 102.40 | 0.0654 | 0.0681 | 0.0668 | 0.0394 |
| 256.00 | 0.1190 | 0.1274 | 0.1232 | 0.0958 |
| 640.00 | 0.2596 | 0.2716 | 0.2656 | 0.2382 |
| 1600.00 | 0.5838 | 0.5924 | 0.5881 | 0.5607 |
| 4000.00 | 1.3170 | 1.4010 | 1.3590 | 1.3316 |
| 10000.00 | 2.6860 | 2.8560 | 2.7710 | 2.7436 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 194.7 | 1006.1 | 2864.5 | 191.6 | 887.7 | 2620.7 |
| Standard Deviation | 13.2 | 60.8 | 221.1 | 13.5 | 65.3 | 80.9 |
| Coefficient of Variation(%) | 6.8 | 6.0 | 7.7 | 7.0 | 7.4 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MMP-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 121% with an overall mean recovery of 114%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-42.89 | 31.25 | 18.13 |
Serum/Plasma– Thirty samples from apparently healthy volunteers serum were evaluated for the presence of human MMP-1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: MMP-1
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,
alpha ‑1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
