Human MIP-5/CCL15 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 13.85 pg/ml (50 μl) |
| Standard Curve Range | 31.25~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0090 | 0.0090 | 0.0090 | |
| 31.25 | 0.0130 | 0.0140 | 0.0135 | 0.0045 |
| 62.50 | 0.0230 | 0.0210 | 0.0220 | 0.0130 |
| 125.00 | 0.0540 | 0.0490 | 0.0515 | 0.0425 |
| 250.00 | 0.1670 | 0.1450 | 0.1560 | 0.1470 |
| 500.00 | 0.5270 | 0.5010 | 0.5140 | 0.5050 |
| 1000.00 | 1.6370 | 1.5760 | 1.6065 | 1.5975 |
| 2000.00 | 3.2740 | 3.2740 | 3.2740 | 3.2650 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 68.2 | 262.6 | 718.7 | 47.7 | 214.8 | 644.6 |
| Standard Deviation | 5.4 | 15.6 | 40.3 | 3.8 | 10.1 | 32.8 |
| Coefficient of Variation(%) | 7.9 | 5.9 | 5.6 | 7.9 | 4.7 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MIP-5/CCL15 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 105% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2321.43-15417.02 | 100 | 10905.55 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human MIP-5/CCL15 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: MIP-5/CCL15
CCL15, also called macrophage inflammatory protein 1 delta (MIP-1 delta and Leukotactin-1 (LKN-1), is a CC chemokine with two additional cysteine residues that form a third disulfide bond. CCL15 functions as a chemoattractant for monocytes, T cells, and eosinophils through interactions with CCR1. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1 delta is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells.
