Human Lipoprotein A/LPA enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.11 pg/ml (50 μl)；7.76 pg/ml (10 μl)； |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0070 | 0.0063 | 0.0067 | |
| 13.72 | 0.0173 | 0.0213 | 0.0193 | 0.0127 |
| 41.15 | 0.0419 | 0.0407 | 0.0413 | 0.0347 |
| 123.46 | 0.1194 | 0.1158 | 0.1176 | 0.1110 |
| 370.37 | 0.3628 | 0.3434 | 0.3531 | 0.3465 |
| 1111.11 | 1.0670 | 1.0320 | 1.0495 | 1.0429 |
| 3333.33 | 2.5740 | 2.5650 | 2.5695 | 2.5629 |
| 10000.00 | 3.9935 | 4.0450 | 4.0193 | 4.0126 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 375.0 | 1408.8 | 3317.3 | 371.3 | 1405.8 | 3425.6 |
| Standard Deviation | 10.0 | 36.1 | 99.1 | 12.8 | 69.1 | 226.0 |
| Coefficient of Variation(%) | 2.7 | 2.6 | 3.0 | 3.4 | 4.9 | 6.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Lipoprotein A/LPA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 118% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 288.08-11292.84 | 100 | 2363.67 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Lipoprotein A/LPA in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Lipoprotein A/LPA
The protein encoded by this gene is a serine proteinase that inhibits the activity of tissue-type plasminogen activator I. The encoded protein constitutes a substantial portion of lipoprotein(a) and is proteolytically cleaved, resulting in fragments that attach to atherosclerotic lesions and promote thrombogenesis. Elevated plasma levels of this protein are linked to atherosclerosis. Depending on the individual, the encoded protein contains 2-43 copies of kringle-type domains. The allele represented here contains 15 copies of the kringle-type repeats and corresponds to that found in the reference genome sequence.
