Human Interferon omega-1/IFNW1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.38 pg/ml (50 μl)；1.54 pg/ml (10 μl)； |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0147 | 0.0141 | 0.0144 | |
| 1.37 | 0.0203 | 0.0178 | 0.0191 | 0.0047 |
| 4.12 | 0.0317 | 0.0330 | 0.0324 | 0.0180 |
| 12.35 | 0.0697 | 0.0698 | 0.0698 | 0.0554 |
| 37.04 | 0.1800 | 0.1795 | 0.1798 | 0.1654 |
| 111.11 | 0.5158 | 0.5177 | 0.5168 | 0.5024 |
| 333.33 | 1.4350 | 1.5300 | 1.4825 | 1.4681 |
| 1000.00 | 3.7290 | 3.7800 | 3.7545 | 3.7401 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 24.4 | 106.3 | 299.9 | 24.0 | 108.0 | 333.4 |
| Standard Deviation | 1.9 | 6.1 | 23.9 | 0.4 | 3.2 | 3.7 |
| Coefficient of Variation(%) | 8.0 | 5.8 | 8.0 | 1.6 | 2.9 | 1.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Interferon omega-1/IFNW1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 109% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.27 | 18.75 | 0.20 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Interferon omega-1/IFNW1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Interferon omega-1/IFNW1
Interferon-omega (IFN-omega), a type I interferon, is a monomeric glycoprotein distantly related in structure to IFN-alpha and IFN-beta, but unrelated to IFN-gamma. The human type I interferon receptor complex, which mediates the biological activity of IFN-alpha and IFN-beta, also binds IFN-omega. The complex is composed of a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand binding and signal transduction. IFN-omega is secreted by virus-infected leukocytes.
