Human Interferon alpha-2/IFNA2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.49 pg/ml (50 μl)；15.27 pg/ml (10 μl)； |
| Standard Curve Range | 20.48 ~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0097 | 0.0098 | 0.0098 | |
| 20.48 | 0.0136 | 0.0136 | 0.0136 | 0.0039 |
| 51.20 | 0.0227 | 0.0225 | 0.0226 | 0.0129 |
| 128.00 | 0.0527 | 0.0527 | 0.0527 | 0.0430 |
| 320.00 | 0.1465 | 0.1459 | 0.1462 | 0.1365 |
| 800.00 | 0.4221 | 0.4055 | 0.4138 | 0.4041 |
| 2000.00 | 1.2170 | 1.2100 | 1.2135 | 1.2038 |
| 5000.00 | 3.0470 | 3.0530 | 3.0500 | 3.0403 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 191.8 | 741.7 | 2019.6 | 185.7 | 703.5 | 1942.1 |
| Standard Deviation | 5.3 | 17.8 | 43.8 | 4.6 | 15.3 | 56.4 |
| Coefficient of Variation(%) | 2.8 | 2.4 | 2.2 | 2.5 | 2.2 | 2.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Interferon alpha-2/IFNA2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 117%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-11.25 | 6.25 | 11.25 |
Serum/Plasma– Thirty samples from apparently healthy volunteers were evaluated for the presence of human Interferon alpha-2/IFNA2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Interferon alpha-2/IFNA2
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
