Human IL-6R enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.05 ng/ml (50 μl)；0.56 ng/ml (10 μl)； |
| Standard Curve Range | 0.41~100 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0086 | 0.0083 | |
| 0.41 | 0.0145 | 0.0145 | 0.0145 | 0.0063 |
| 1.02 | 0.0287 | 0.0276 | 0.0282 | 0.0199 |
| 2.56 | 0.0740 | 0.0729 | 0.0735 | 0.0652 |
| 6.40 | 0.2382 | 0.2381 | 0.2382 | 0.2299 |
| 16.00 | 0.8391 | 0.8159 | 0.8275 | 0.8193 |
| 40.00 | 2.2770 | 2.1820 | 2.2295 | 2.2213 |
| 100.00 | 3.8800 | 3.9150 | 3.8975 | 3.8893 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 1.8 | 8.5 | 29.5 | 1.8 | 8.6 | 30.6 |
| Standard Deviation | 0.0 | 0.2 | 1.1 | 0.0 | 0.2 | 0.7 |
| Coefficient of Variation(%) | 2.2 | 2.2 | 3.7 | 2.4 | 2.3 | 2.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-6R into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 116% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 16.73-65.67 | 100 | 36.52 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-6R in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-6R
