Human IL-34 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.33 pg/ml (50 μl)；7.71 pg/ml (10 μl)； |
| Standard Curve Range | 20.48~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0082 | 0.0085 | 0.0084 | |
| 20.48 | 0.0274 | 0.0267 | 0.0271 | 0.0187 |
| 51.20 | 0.0575 | 0.0565 | 0.0570 | 0.0487 |
| 128.00 | 0.1349 | 0.1291 | 0.1320 | 0.1237 |
| 320.00 | 0.3167 | 0.3152 | 0.3160 | 0.3076 |
| 800.00 | 0.7983 | 0.7546 | 0.7765 | 0.7681 |
| 2000.00 | 1.9480 | 1.8120 | 1.8800 | 1.8717 |
| 5000.00 | 3.6500 | 3.6880 | 3.6690 | 3.6607 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 72.6 | 395.1 | 1347.7 | 75.2 | 437.4 | 1388.9 |
| Standard Deviation | 4.5 | 15.9 | 58.5 | 4.8 | 32.7 | 82.4 |
| Coefficient of Variation(%) | 6.1 | 4.0 | 4.3 | 6.4 | 7.5 | 5.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-34 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 111% with an overall mean recovery of 94%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.20-3.88 | 100 | 2.22 |
Serum/Plasma– Thirty samples from apparently healthy volunteers serum were evaluated for the presence of human IL-34 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-34
Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N‑linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level. IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit‑macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.
