Human IL-31 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.4 pg/ml (50 μl)；2.68 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0165 | 0.0192 | 0.0179 | |
| 1.37 | 0.0268 | 0.0277 | 0.0273 | 0.0094 |
| 4.12 | 0.0450 | 0.0391 | 0.0421 | 0.0242 |
| 12.35 | 0.0782 | 0.0770 | 0.0776 | 0.0598 |
| 37.04 | 0.2055 | 0.1949 | 0.2002 | 0.1824 |
| 111.11 | 0.5257 | 0.5334 | 0.5296 | 0.5117 |
| 333.33 | 1.4740 | 1.5710 | 1.5225 | 1.5047 |
| 1000.00 | 3.7040 | 3.7270 | 3.7155 | 3.6977 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 305.1 | 104.3 | 24.3 | 338.2 | 112.0 | 23.2 |
| Standard Deviation | 12.7 | 4.2 | 1.3 | 18.8 | 2.8 | 1.5 |
| Coefficient of Variation(%) | 4.2 | 4.0 | 5.2 | 5.6 | 2.5 | 6.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-31 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 98% to 124% with an overall mean recovery of 114%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.34 | 69 | 1.16 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-31 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-31
Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein. The mature region shows four alpha -helices which would be expected to show a typical up‑up‑down‑down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region. IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL‑31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ). In the complex, IL-31 directly binds to GPL, not OSM R. IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway. Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable. The IL-31 receptor is constitutively expressed by keratinocytes and up‑regulated by IFN-gamma on monocytes. Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis.
