Human IL-29/IFN-Iambda 1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.15 pg/ml (50 μl)；0.56 pg/ml (10 μl)； |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0089 | 0.0087 | 0.0088 | |
| 1.37 | 0.0154 | 0.0144 | 0.0149 | 0.0061 |
| 4.12 | 0.0311 | 0.0299 | 0.0305 | 0.0217 |
| 12.35 | 0.0837 | 0.0752 | 0.0795 | 0.0707 |
| 37.04 | 0.2048 | 0.2172 | 0.2110 | 0.2022 |
| 111.11 | 0.5411 | 0.5202 | 0.5307 | 0.5219 |
| 333.33 | 1.6650 | 1.5420 | 1.6035 | 1.5947 |
| 1000.00 | 3.3770 | 3.2140 | 3.2955 | 3.2867 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 30.3 | 151.2 | 497.0 | 23.7 | 115.1 | 364.0 |
| Standard Deviation | 1.9 | 9.7 | 37.0 | 1.3 | 3.5 | 7.8 |
| Coefficient of Variation(%) | 6.2 | 6.4 | 7.4 | 5.6 | 3.0 | 2.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human IL-29/IFN-Iambda 1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 110% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | n.d.-3.18 | 81.3 | 0.78 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-29/IFN-Iambda 1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-29/IFN-Iambda 1
IFN-lambda 1 (IL-29) is a member of the type III interferon family. IFN-lambda 1 signals through the heterodimeric receptor complex of IFNλ receptor 1 (IFNLR1) and IL-10 receptor subunit-β (IL-10RB). When IFN-lambda 1 binds to the receptor complex, Jak1 and Tyk2 will be activated and lead to subsequent IFN-λR1 tyrosine phosphorylation, and activate STAT1 and STAT2. IFN-lambda 1 regulates immunity in anti-infective and autoimmune diseases. IFN-lambda 1/IL-29 protein, Human is a recombinant human IFN-lambda 1 (G20-T200) produced by E. coli without any tag.
