Human IL-22 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.03 pg/ml (50 μl)；0.21 pg/ml (10 μl) |
| Standard Curve Range | 0.69~500 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0285 | 0.0284 | 0.0285 | |
| 0.69 | 0.0412 | 0.0401 | 0.0407 | 0.0122 |
| 2.06 | 0.0647 | 0.0618 | 0.0633 | 0.0348 |
| 6.17 | 0.1402 | 0.1387 | 0.1395 | 0.1110 |
| 18.52 | 0.3698 | 0.3546 | 0.3622 | 0.3338 |
| 55.56 | 0.9875 | 0.9855 | 0.9865 | 0.9581 |
| 166.67 | 2.4529 | 2.3547 | 2.4038 | 2.3754 |
| 500.00 | 4.1289 | 4.1300 | 4.1295 | 4.1010 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 10.7 | 48.9 | 172.6 | 10.6 | 48.6 | 176.8 |
| Standard Deviation | 0.3 | 1.4 | 5.5 | 0.5 | 2.0 | 8.6 |
| Coefficient of Variation(%) | 2.7 | 2.9 | 3.2 | 4.6 | 4.2 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-22 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 117% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 6.48-76 | 100 | 27.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-22 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-22
IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
