Human IL-18R1/CD18a enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.43 pg/ml (50 μl)；1.33 pg/ml (10 μl)； |
| Standard Curve Range | 2.05 ~500 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0574 | 0.0527 | 0.0551 | |
| 2.05 | 0.0849 | 0.0757 | 0.0803 | 0.0253 |
| 5.12 | 0.1180 | 0.1128 | 0.1154 | 0.0604 |
| 12.80 | 0.2140 | 0.2044 | 0.2092 | 0.1542 |
| 32.00 | 0.4363 | 0.4257 | 0.4310 | 0.3760 |
| 80.00 | 0.9716 | 0.9214 | 0.9465 | 0.8915 |
| 200.00 | 2.0860 | 1.9970 | 2.0415 | 1.9865 |
| 500.00 | 3.8860 | 3.8890 | 3.8875 | 3.8325 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 7.3 | 39.8 | 135.0 | 11.0 | 52.9 | 174.7 |
| Standard Deviation | 0.6 | 1.5 | 6.5 | 0.3 | 1.4 | 2.6 |
| Coefficient of Variation(%) | 7.9 | 3.7 | 4.8 | 3.1 | 2.6 | 1.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-18R1/CD218a into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 119% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 49.52-161.17 | 100 | 94.95 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-18R1/CD218a in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-18R1/CD18a
IL-18 receptor is composed of an alpha and a beta subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor alpha chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis. The H44 antibody is specific for IL-18 receptor alpha chain and has been reported to block IL-18 binding to the receptor and to be useful for the identification of IL-18 receptor by flow cytometry and IHC.
