Human IL-17RA/CD217 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.50 pg/ml (50 μl)；11.14 pg/ml (10 μl)； |
| Standard Curve Range | 78.13 ~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0557 | 0.0552 | 0.0555 | |
| 78.13 | 0.1731 | 0.1738 | 0.1735 | 0.1180 |
| 156.25 | 0.2638 | 0.2971 | 0.2805 | 0.2250 |
| 312.50 | 0.4855 | 0.5111 | 0.4983 | 0.4429 |
| 625.00 | 0.8464 | 0.9013 | 0.8739 | 0.8184 |
| 1250.00 | 1.4570 | 1.4230 | 1.4400 | 1.3846 |
| 2500.00 | 2.1660 | 2.1390 | 2.1525 | 2.0971 |
| 5000.00 | 3.0440 | 3.1250 | 3.0845 | 3.0291 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 46.8 | 366.5 | 1128.6 | 47.2 | 358.4 | 1196.9 |
| Standard Deviation | 3.5 | 17.0 | 46.3 | 2.1 | 17.4 | 77.3 |
| Coefficient of Variation(%) | 7.6 | 4.6 | 4.1 | 4.4 | 4.8 | 6.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-17RA/CD217 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 107% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 900.28-3172.52 | 94 | 1705.32 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-17RA/CD217 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-17RA/CD217
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
