Human IL-12B/IL-12 p40 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.29 pg/ml (50 μl)；1.84 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0228 | 0.0235 | 0.0232 | |
| 1.37 | 0.0310 | 0.0304 | 0.0307 | 0.0076 |
| 4.12 | 0.0481 | 0.0481 | 0.0481 | 0.0250 |
| 12.35 | 0.0945 | 0.0931 | 0.0938 | 0.0707 |
| 37.04 | 0.2360 | 0.2307 | 0.2334 | 0.2102 |
| 111.11 | 0.6390 | 0.6322 | 0.6356 | 0.6125 |
| 333.33 | 1.7120 | 1.6570 | 1.6845 | 1.6614 |
| 1000.00 | 3.8910 | 3.8380 | 3.8645 | 3.8414 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 19.4 | 104.0 | 339.2 | 19.3 | 101.1 | 309.6 |
| Standard Deviation | 0.7 | 4.3 | 14.4 | 1.3 | 5.4 | 20.1 |
| Coefficient of Variation(%) | 3.4 | 4.1 | 4.2 | 6.6 | 5.4 | 6.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-12B p40 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 117% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 25.15-193.73 | 100 | 80.27 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-12B p40
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
