Human I-309/CCL1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 13.37 pg/ml (50 μl)；45.82 pg/ml (10 μl)； |
| Standard Curve Range | 46.66~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0214 | 0.0182 | 0.0198 | |
| 46.66 | 0.0438 | 0.0447 | 0.0443 | 0.0245 |
| 77.76 | 0.0743 | 0.0819 | 0.0781 | 0.0583 |
| 129.60 | 0.1669 | 0.1777 | 0.1723 | 0.1525 |
| 216.00 | 0.4113 | 0.4199 | 0.4156 | 0.3958 |
| 360.00 | 0.8925 | 0.9650 | 0.9288 | 0.9090 |
| 600.00 | 1.8230 | 2.0240 | 1.9235 | 1.9037 |
| 1000.00 | 3.1680 | 3.2090 | 3.1885 | 3.1687 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 21.5 | 123.2 | 374.9 | 20.5 | 116.2 | 338.7 |
| Standard Deviation | 1.5 | 9.8 | 25.2 | 1.5 | 9.3 | 21.1 |
| Coefficient of Variation(%) | 7.0 | 8.0 | 6.7 | 7.3 | 8.0 | 6.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human I-309/CCL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 119% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d. | 0 | n.d. |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human I-309/CCL1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: I-309/CCL1
Mouse TCA-3 is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.
TCA-3 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of TCA‑3 contains one potential N-linked glycosylation site. Mouse TCA-3 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta, and JE. TCA-3 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. TCA-3 has been shown to chemoattract T cells.
