Human Hemoglobin subunit alpha/HBA1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 9.11 pg/ml (50 μl)；28.56 pg/ml (10 μl)； |
| Standard Curve Range | 68.59~50000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0078 | 0.0079 | |
| 68.59 | 0.0139 | 0.0142 | 0.0141 | 0.0062 |
| 205.76 | 0.0293 | 0.0308 | 0.0301 | 0.0222 |
| 617.28 | 0.0938 | 0.0880 | 0.0909 | 0.0831 |
| 1851.85 | 0.3062 | 0.2938 | 0.3000 | 0.2922 |
| 5555.56 | 1.0950 | 1.1060 | 1.1005 | 1.0927 |
| 16666.67 | 2.5210 | 2.4460 | 2.4835 | 2.4757 |
| 50000.00 | 3.9190 | 3.8470 | 3.8830 | 3.8752 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 945.9 | 4900.6 | 14097.9 | 1278.0 | 5808.4 | 16647.0 |
| Standard Deviation | 72.9 | 355.0 | 1048.4 | 88.4 | 144.2 | 638.3 |
| Coefficient of Variation(%) | 7.7 | 7.2 | 7.4 | 6.9 | 2.5 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Hemoglobin subunit alpha/HBA1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 122% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 78.22-476.01 | 100 | 193.92 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Hemoglobin subunit alpha/HBA1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Hemoglobin subunit alpha/HBA1
Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues.The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.
