Human Granzyme K enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.36 pg/ml (50 μl)；2.44 pg/ml (10 μl)； |
| Standard Curve Range | 4.10~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0260 | 0.0248 | 0.0254 | |
| 4.10 | 0.0598 | 0.0552 | 0.0575 | 0.0321 |
| 10.24 | 0.1089 | 0.1120 | 0.1105 | 0.0851 |
| 25.60 | 0.2159 | 0.2080 | 0.2120 | 0.1866 |
| 64.00 | 0.5337 | 0.5264 | 0.5301 | 0.5047 |
| 160.00 | 1.1840 | 1.1190 | 1.1515 | 1.1261 |
| 400.00 | 2.3040 | 2.1600 | 2.2320 | 2.2066 |
| 1000.00 | 3.7910 | 3.7800 | 3.7855 | 3.7601 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 17.2 | 98.6 | 282.3 | 17.2 | 98.8 | 319.5 |
| Standard Deviation | 0.9 | 5.6 | 22.0 | 0.5 | 4.0 | 22.0 |
| Coefficient of Variation(%) | 5.2 | 5.7 | 7.8 | 2.8 | 4.1 | 6.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Granzyme K into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 98% to 127% with an overall mean recovery of 119%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.63-35.25 | 100 | 11.74 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Granzyme K in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Granzyme K
The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K (GMZK), also designated granzyme-3 or NK-tryptase-2 (NK-TRYP-2), contains one peptidase S1 domain. Granzyme K is a serine protease localizing to the granules of natural killer cells and cytotoxic T lymphocytes. It is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.
