Human FGL1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 10.64 pg/ml (50 μl) |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0554 | 0.0591 | 0.0591 | |
| 156.25 | 0.1239 | 0.1208 | 0.1224 | 0.0633 |
| 312.50 | 0.1804 | 0.1823 | 0.1814 | 0.1223 |
| 625.00 | 0.3132 | 0.3047 | 0.3090 | 0.2499 |
| 1250.00 | 0.5540 | 0.5687 | 0.5614 | 0.5023 |
| 2500.00 | 1.0070 | 1.0420 | 1.0245 | 0.9654 |
| 5000.00 | 1.8910 | 1.9030 | 1.8970 | 1.8379 |
| 10000.00 | 3.1480 | 3.2320 | 3.1900 | 3.1309 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 199.2 | 1022.8 | 3144.4 | 187.7 | 934.2 | 2979.1 |
| Standard Deviation | 10.0 | 44.6 | 147.4 | 7.4 | 38.6 | 131.6 |
| Coefficient of Variation(%) | 5.0 | 4.4 | 4.7 | 3.9 | 4.1 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human FGL1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 120% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 43.10-218.06 | 100 | 104.27 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human FGL1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: FGL1
Hepassocin, also known as hepatocyte-derived fibrinogen-related protein (HFREP-1), is a liver specific secreted protein containing one fibrinogen C-terminal domain. It exists as a homodimer and is reported to have hepatocyte mitogenic activity .
