Human DPP4/CD26/DPPⅣ enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50 μl)；0.11 ng/ml (10 μl)； |
| Standard Curve Range | 4.69~300 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 1 |
| Detectable sample | serum, plasma |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0052 | 0.0054 | 0.0053 | |
| 4.69 | 0.0727 | 0.0724 | 0.0726 | 0.0673 |
| 9.38 | 0.1449 | 0.1551 | 0.1500 | 0.1447 |
| 18.75 | 0.2938 | 0.2989 | 0.2964 | 0.2911 |
| 37.50 | 0.5731 | 0.5777 | 0.5754 | 0.5701 |
| 75.00 | 1.1270 | 1.1430 | 1.1350 | 1.1297 |
| 150.00 | 2.0070 | 2.0590 | 2.0330 | 2.0277 |
| 300.00 | 3.2640 | 3.4690 | 3.3665 | 3.3612 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 5.7 | 29.5 | 91.6 | 5.8 | 32.8 | 101.4 |
| Standard Deviation | 0.2 | 0.9 | 4.2 | 0.2 | 0.9 | 1.9 |
| Coefficient of Variation(%) | 3.4 | 2.9 | 4.6 | 2.8 | 3.0 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human DPP4/CD26/DPPIV into health Human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 213.09-476.01 | 100 | 384.53 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Human DPP4/CD26/DPPIV in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: DPP4/CD26/DPPⅣ
CD26 is encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
