Human CXCL6/GCP-2 enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 4.34 pg/ml (50 μl)；13.58 pg/ml (10 μl)； |
| Standard Curve Range | 31.25~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0073 | 0.0077 | 0.0075 | |
| 31.25 | 0.0185 | 0.0197 | 0.0191 | 0.0116 |
| 62.50 | 0.0450 | 0.0437 | 0.0444 | 0.0369 |
| 125.00 | 0.1488 | 0.1519 | 0.1504 | 0.1429 |
| 250.00 | 0.5995 | 0.6087 | 0.6041 | 0.5966 |
| 500.00 | 1.6640 | 1.7040 | 1.6840 | 1.6765 |
| 1000.00 | 2.9450 | 2.9480 | 2.9465 | 2.9390 |
| 2000.00 | 3.8530 | 3.8660 | 3.8595 | 3.8520 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 139.4 | 425.2 | 854.1 | 107.3 | 323.6 | 710.4 |
| Standard Deviation | 6.7 | 8.8 | 16.6 | 3.6 | 19.4 | 26.5 |
| Coefficient of Variation(%) | 4.8 | 2.1 | 1.9 | 3.4 | 6.0 | 3.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL6/GCP-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 118% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 18.88-92.95 | 100 | 55.65 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL6/GCP-2 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CXCL6/GCP-2
GCP-2 (granulocyte chemotactic protein-2) is a CXC chemokine. Among human CXC chemokines, GCP-2 is most closely related to ENA-78. The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity, suggesting the two genes may have originated from a recent gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The mouse protein designated GCP-2, because of its amino acid sequence similarity (60%) to human GCP-2, is identical to the LIX protein sequence.
