Human CXCL2/MIP2/GRO beta enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.31 pg/ml (50 μl)；0.89 pg/ml (10 μl)； |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0069 | 0.0063 | 0.0066 | |
| 6.86 | 0.0100 | 0.0096 | 0.0098 | 0.0032 |
| 20.58 | 0.0166 | 0.0155 | 0.0161 | 0.0095 |
| 61.73 | 0.0397 | 0.0389 | 0.0393 | 0.0327 |
| 185.19 | 0.1299 | 0.1234 | 0.1267 | 0.1201 |
| 555.56 | 0.5090 | 0.4817 | 0.4954 | 0.4888 |
| 1666.67 | 1.7460 | 1.7880 | 1.7670 | 1.7604 |
| 5000.00 | 3.3580 | 3.2720 | 3.3150 | 3.3084 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 37.0 | 198.3 | 824.2 | 41.6 | 203.9 | 802.0 |
| Standard Deviation | 1.8 | 10.5 | 38.3 | 0.9 | 7.1 | 34.8 |
| Coefficient of Variation(%) | 4.7 | 5.3 | 4.7 | 2.2 | 3.5 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL2/MIP2/GRO beta into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 110% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Male serum | 15 | 246.13-944.54 | 100 | 518.96 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CXCL2/MIP2/GRO in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CXCL2/MIP2/GRO beta
CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
