Human CXCL16 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.58 pg/ml (50 μl)；64.57 pg/ml (10 μl)； |
| Standard Curve Range | 68.59 ~50000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 1 |
| Detectable sample | serum, plasma |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0141 | 0.0141 | 0.0141 | |
| 68.59 | 0.0289 | 0.0256 | 0.0273 | 0.0132 |
| 205.76 | 0.0525 | 0.0525 | 0.0525 | 0.0384 |
| 617.28 | 0.1262 | 0.1339 | 0.1301 | 0.1160 |
| 1851.85 | 0.3452 | 0.3478 | 0.3465 | 0.3324 |
| 5555.56 | 0.9408 | 0.8781 | 0.9095 | 0.8954 |
| 16666.67 | 1.9910 | 1.8870 | 1.9390 | 1.9249 |
| 50000.00 | 2.9910 | 3.0280 | 3.0095 | 2.9954 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 0.7 | 5.0 | 17.9 | 0.8 | 4.8 | 16.1 |
| Standard Deviation | 0.0 | 0.4 | 1.3 | 0.0 | 0.3 | 1.1 |
| Coefficient of Variation(%) | 6.8 | 7.3 | 7.3 | 1.9 | 5.8 | 6.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL16 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 72% to 108% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.07-7.86 | 100 | 5.15 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CXCL16 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CXCL16
CXC chemokine ligand 16 (CXCL16) is a type I membrane protein that has a non-ELR CXC chemokine domain in its extracellular region. Fractalkine (CX3CL1) and CXCL16 constitute the only two transmembrane chemokines. Mouse and human CXCL16 share 70% amino acid sequence similarity within their chemokine domains and 49% overall amino acid sequence identity.
