Human CXCL13/BCA-1 DTSet enzyme-linked immunoassay kit
| Specification | 96*5 Test；96T*15 Test |
|---|---|
| Standard Curve Range | 7.81 pg/ml -500 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | |
| Sample Volume | 50 μl |
| Type | Not Ready-to-Use |
| Test Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0127 | 0.0135 | 0.0131 | |
| 7.81 | 0.0563 | 0.0629 | 0.0596 | 0.0465 |
| 15.63 | 0.1188 | 0.1026 | 0.1107 | 0.0976 |
| 31.25 | 0.2504 | 0.2681 | 0.2593 | 0.2462 |
| 62.50 | 0.5489 | 0.5339 | 0.5414 | 0.5283 |
| 125.00 | 1.2540 | 1.3080 | 1.2810 | 1.2679 |
| 250.00 | 2.5910 | 2.8150 | 2.7030 | 2.6899 |
| 500.00 | 4.2870 | 4.1706 | 4.2288 | 4.2157 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DTSet Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: YOUKE Life, Catalog # DSEP01. Plate Sealers: YOUKE Life, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2μm filtered . YOUKE Life, Catalog # DSCB01.
- Blocking Buffer: YOUKE Life, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween® 20 in PBS, pH 7.2-7.4. YOUKE Life, Catalog # DSWB01.
- Assay Buffer: 0.5% BSA，0.05% Tween® 20，PBS Solution.YOUKE Life, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. YOUKE Life, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. YOUKE Life, Catalog # DSSS01.
Product Data Sheet
Background: CXCL13/BCA-1
CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.
