Human CX3CL1/Fractalking enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 7.47 pg/ml (50 μl)；16.03 pg/ml (10 μl)； |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0138 | 0.0133 | 0.0136 | |
| 156.25 | 0.0427 | 0.0364 | 0.0396 | 0.0260 |
| 312.50 | 0.0842 | 0.0711 | 0.0777 | 0.0641 |
| 625.00 | 0.1921 | 0.1773 | 0.1847 | 0.1712 |
| 1250.00 | 0.5697 | 0.4696 | 0.5197 | 0.5061 |
| 2500.00 | 1.3220 | 1.2500 | 1.2860 | 1.2725 |
| 5000.00 | 2.5240 | 2.4520 | 2.4880 | 2.4745 |
| 10000.00 | 3.5110 | 3.5180 | 3.5145 | 3.5010 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 285.8 | 1505.4 | 4447.7 | 294.6 | 1388.2 | 4357.8 |
| Standard Deviation | 21.7 | 80.1 | 223.3 | 12.3 | 74.3 | 153.6 |
| Coefficient of Variation(%) | 7.6 | 5.3 | 5.0 | 4.2 | 5.4 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human CX3CL1/Fractalking into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 79% to 128% with an overall mean recovery of 114%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 516.05-2692.08 | 100 | 1625.88 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CX3CL1/Fractalking in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CX3CL1/Fractalking
Fractalkine（CX3CL1）是CX3C类趋化因子中唯一成员，由373个氨基酸构成，存在膜结合型和分泌型两种形式。其膜结合型由趋化结构域、粘蛋白样茎状结构、跨膜域及胞内域组成，可被炎症因子诱导表达；分泌型通过蛋白水解生成，具有趋化活性。在哺乳动物脑、肺、心脏等器官中均有表达，神经元及胶质细胞可分泌该蛋白。
