Human CD200 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.55 pg/ml (50 μl)；4.24 pg/ml (10 μl)； |
| Standard Curve Range | 31.25~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0197 | 0.0199 | 0.0198 | |
| 31.25 | 0.0989 | 0.0996 | 0.0993 | 0.0795 |
| 62.50 | 0.1858 | 0.1874 | 0.1866 | 0.1668 |
| 125.00 | 0.3507 | 0.3609 | 0.3558 | 0.3360 |
| 250.00 | 0.7370 | 0.6479 | 0.6925 | 0.6727 |
| 500.00 | 1.3060 | 1.2280 | 1.2670 | 1.2472 |
| 1000.00 | 2.3000 | 2.1670 | 2.2335 | 2.2137 |
| 2000.00 | 3.7050 | 3.6340 | 3.6695 | 3.6497 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 47.5 | 227.2 | 648.6 | 45.6 | 220.5 | 665.2 |
| Standard Deviation | 2.2 | 6.8 | 22.6 | 2.1 | 10.0 | 19.0 |
| Coefficient of Variation(%) | 4.6 | 3.0 | 3.5 | 4.5 | 4.5 | 2.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD200 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 110% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 187.6-436.31 | 100 | 315.16 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD200 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD200
CD200, also known as OX-2, is a 45 kDa transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily. The human CD200 cDNA encodes a 278 amino acid (aa) precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain. A splice variant of CD200 has been described and has a truncated cytoplasmic tail. Within the ECD, human CD200 shares 76% aa sequence identity with mouse and rat CD200. CD200 is widely but not ubiquitously expressed. Its receptor (CD200R) is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of CD200. CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders. CD200 and CD200R associate via their respective N-terminal Ig-like domains. In myeloid cells, CD200R initiates inhibitory signals following receptor‑ligand contact. In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway. Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial. Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase. Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation.
