Human CD147/EMMPRIN enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.44 pg/ml (50 μl)；3.63 pg/ml (10 μl) |
| Standard Curve Range | 5.49~4000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0094 | 0.0094 | 0.0094 | |
| 5.49 | 0.0292 | 0.0303 | 0.0298 | 0.0204 |
| 16.46 | 0.0711 | 0.0747 | 0.0729 | 0.0635 |
| 49.38 | 0.2033 | 0.2031 | 0.2032 | 0.1938 |
| 148.15 | 0.5809 | 0.6104 | 0.5957 | 0.5863 |
| 444.44 | 1.4780 | 1.4830 | 1.4805 | 1.4711 |
| 1333.33 | 2.8760 | 2.7790 | 2.8275 | 2.8181 |
| 4000.00 | 3.9430 | 3.8660 | 3.9045 | 3.8951 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 112.4 | 569.7 | 1538.8 | 101.4 | 480.9 | 1376.3 |
| Standard Deviation | 4.0 | 21.3 | 94.7 | 4.6 | 28.0 | 80.6 |
| Coefficient of Variation(%) | 3.6 | 3.7 | 6.2 | 4.5 | 5.8 | 5.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD147/EMMPRIN into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 103% with an overall mean recovery of 86%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 139.08-781.71 | 100 | 234.71 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD147/EMMPRIN in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD147/EMMPRIN
The name EMMPRIN reflects this proteins extracellular metalloproteinase inducer activity. It is a cell surface protein with two extracellular Ig domains, a transmembrane domain, and a cytoplasmic domain. Its expression is often elevated in human tumor cells, which in turn promotes MMP-1, MMP-2 and MMP-3 production in melanoma cells and MMP-2 and MMP-9 production in breast cancer cells. It interacts with several types of molecules such as integrins, caveolin-1 and MCT1. TRA-1-85 is an epitope within EMMPRIN.
