Human CD127/IL-7 R alpha enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 10.55 pg/ml (10 μl) |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0640 | 0.0630 | 0.0635 | |
| 156.25 | 0.1851 | 0.1668 | 0.1760 | 0.1125 |
| 312.50 | 0.4100 | 0.3861 | 0.3981 | 0.3346 |
| 625.00 | 0.7055 | 0.6823 | 0.6939 | 0.6304 |
| 1250.00 | 1.2610 | 1.2630 | 1.2620 | 1.1985 |
| 2500.00 | 2.0830 | 2.0410 | 2.0620 | 1.9985 |
| 5000.00 | 3.1730 | 3.1000 | 3.1365 | 3.0730 |
| 10000.00 | 4.1100 | 4.0396 | 4.0748 | 4.0113 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 196.2 | 1212.5 | 3366.8 | 200.9 | 1152.1 | 3404.0 |
| Standard Deviation | 6.7 | 40.4 | 193.6 | 6.5 | 57.4 | 147.8 |
| Coefficient of Variation(%) | 3.4 | 3.3 | 5.8 | 3.2 | 5.0 | 4.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Human CD127/IL-7 R alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 74% to 107% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4551.82-11441.82 | 100 | 7455.88 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Human CD127/IL-7 R alpha in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CD127/IL-7 R alpha
Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.
