Human CCL17/TARC enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 5.67 pg/ml (50 μl)；23.55 pg/ml (10 μl)； |
| Standard Curve Range | 15.63~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0095 | 0.0089 | 0.0092 | |
| 15.63 | 0.0154 | 0.0164 | 0.0159 | 0.0067 |
| 31.25 | 0.0316 | 0.0331 | 0.0324 | 0.0232 |
| 62.50 | 0.1050 | 0.1019 | 0.1035 | 0.0943 |
| 125.00 | 0.3403 | 0.3721 | 0.3562 | 0.3470 |
| 250.00 | 1.0840 | 1.0790 | 1.0815 | 1.0723 |
| 500.00 | 2.7800 | 2.5100 | 2.6450 | 2.6358 |
| 1000.00 | 4.1525 | 3.9872 | 4.0699 | 4.0607 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 34.6 | 139.7 | 383.4 | 26.0 | 123.2 | 325.1 |
| Standard Deviation | 2.1 | 6.4 | 26.2 | 0.5 | 2.8 | 17.0 |
| Coefficient of Variation(%) | 6.2 | 4.6 | 6.8 | 2.1 | 2.3 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL17/TARC into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 100% to 128% with an overall mean recovery of 115%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 28.6-272.53 | 100 | 110.93 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL17/TARC in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CCL17/TARC
CCL17/TARC is a chemokine that interacts with CCR4 and CCR8 to induce the chemoattraction of activated Th2 cells, basophils, and NK cells. It is produced by thymic dendritic cells, lymph node dendritic cells, monocytes, CD4+ T cells, keratinocytes, fibroblasts, bronchial epithelial cells, and Reed-Sternberg cells. CCL17 promotes Th2 cell recruitment to sites of inflammation and induces platelet aggregation and degranulation.
