Human CCL16/LEC enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (10 μl) |
| Standard Curve Range | 0.78~50 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 1 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0054 | 0.0054 | 0.0054 | |
| 0.78 | 0.1235 | 0.1153 | 0.1194 | |
| 0.1140 | 1.56 | 0.2368 | 0.2407 | 0.2388 |
| 0.2334 | 3.13 | 0.4504 | 0.4531 | 0.4518 |
| 0.4464 | 6.25 | 0.8144 | 0.8275 | 0.8210 |
| 0.8156 | 12.50 | 1.4330 | 1.5100 | 1.4715 |
| 1.4661 | 25.00 | 2.4360 | 2.4490 | 2.4425 |
| 2.4371 | 50.00 | 3.5390 | 3.6880 | 3.6135 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.3 | 6.9 | 17.2 | 1.5 | 6.4 | 16.2 |
| Standard Deviation | 0.1 | 0.3 | 0.8 | 0.1 | 0.3 | 0.6 |
| Coefficient of Variation(%) | 4.3 | 4.8 | 4.5 | 4.1 | 4.7 | 3.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL16/LEC into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 109% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.33-13.11 | 100 | 8.66 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CCL16/LEC in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CCL16/LEC
CCL16 gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL16 displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10.
