Human CCL11/Eotaxin enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 4.73 pg/ml (50 μl)；8.97 pg/ml (10 μl)； |
| Standard Curve Range | 62.5~4000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0179 | 0.0182 | 0.0181 | |
| 62.50 | 0.0510 | 0.0524 | 0.0517 | 0.0337 |
| 125.00 | 0.0924 | 0.0940 | 0.0932 | 0.0752 |
| 250.00 | 0.1999 | 0.2008 | 0.2004 | 0.1823 |
| 500.00 | 0.4787 | 0.4712 | 0.4750 | 0.4569 |
| 1000.00 | 1.1480 | 1.1570 | 1.1525 | 1.1345 |
| 2000.00 | 2.7190 | 2.5540 | 2.6365 | 2.6185 |
| 4000.00 | 4.4816 | 4.4996 | 4.4906 | 4.4726 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 111.0 | 495.8 | 1676.5 | 100.0 | 402.4 | 1352.0 |
| Standard Deviation | 6.2 | 23.4 | 93.6 | 4.1 | 19.1 | 74.6 |
| Coefficient of Variation(%) | 5.6 | 4.7 | 5.6 | 4.1 | 4.7 | 5.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL11/Eotaxin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 104% to 120% with an overall mean recovery of 117%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | n.d.-151.13 | 57 | 38.4 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL11/Eotaxin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CCL11/Eotaxin
CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.
