Human Brain-derived tau/BD-tau enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 10.15 pg/ml (50 μl)；56.37 pg/ml (10 μl)； |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0386 | 0.0385 | 0.0386 | |
| 156.25 | 0.1011 | 0.1031 | 0.1021 | 0.0636 |
| 312.50 | 0.1564 | 0.1648 | 0.1606 | 0.1221 |
| 625.00 | 0.2924 | 0.2871 | 0.2898 | 0.2512 |
| 1250.00 | 0.5086 | 0.5633 | 0.5360 | 0.4974 |
| 2500.00 | 0.9674 | 1.0270 | 0.9972 | 0.9587 |
| 5000.00 | 1.8070 | 1.8870 | 1.8470 | 1.8085 |
| 10000.00 | 3.0960 | 3.0570 | 3.0765 | 3.0380 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 155.0 | 789.1 | 2538.8 | 125.3 | 855.0 | 3000.6 |
| Standard Deviation | 10.1 | 35.8 | 166.2 | 8.4 | 38.8 | 152.3 |
| Coefficient of Variation(%) | 6.5 | 4.5 | 6.5 | 6.7 | 4.5 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Brain-derived tau/BD-tau into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 115% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | n.d.-181.86 | 40 | 57.65 |
Serum/Plasma– Thirty samples from apparently healthy volunteers/mouse serum were evaluated for the presence of human Brain-derived tau/BD-tau in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Brain-derived tau/BD-tau
