Human Amphiregulin/ARGE enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 8.05 pg/ml (50 μl)；49.15 pg/ml (10 μl)； |
| Standard Curve Range | 31.25 ~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0254 | 0.0254 | 0.0254 | |
| 31.25 | 0.0404 | 0.0372 | 0.0388 | 0.0134 |
| 62.50 | 0.0582 | 0.0572 | 0.0577 | 0.0323 |
| 125.00 | 0.0945 | 0.0989 | 0.0967 | 0.0713 |
| 250.00 | 0.2107 | 0.2184 | 0.2146 | 0.1892 |
| 500.00 | 0.5350 | 0.5538 | 0.5444 | 0.5190 |
| 1000.00 | 1.5250 | 1.4390 | 1.4820 | 1.4566 |
| 2000.00 | 3.5120 | 3.5410 | 3.5265 | 3.5011 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 33.5 | 192.6 | 626.7 | 37.0 | 200.0 | 630.3 |
| Standard Deviation | 2.3 | 7.0 | 26.0 | 0.3 | 12.0 | 11.8 |
| Coefficient of Variation(%) | 6.7 | 3.6 | 4.1 | 0.7 | 6.0 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Amphiregulin/AREG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 104% with an overall mean recovery of 90%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1000.69 | 94 | 238.91 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human Amphiregulin/AREG in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Amphiregulin/ARGE
Amphiregulin (AR) is an EGF family growth factor that is released as a soluble protein following proteolytic cleavage of its transmembrane precursor. Amphiregulin is expressed by numerous carcinoma cell lines and epithelial cells of the colon, stomach, breast, ovary and kidney. It acts through ErbB family of receptor to stimulate the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. Amphiregulin can also inhibit the growth of certain tumor cells.
