Human alpha Fetoprotein enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 6.88 pg/ml (50 μl)；32.49 pg/ml (10 μl)； |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0071 | 0.0068 | 0.0070 | |
| 156.25 | 0.0240 | 0.0229 | 0.0235 | 0.0165 |
| 312.50 | 0.0454 | 0.0476 | 0.0465 | 0.0396 |
| 625.00 | 0.1040 | 0.1062 | 0.1051 | 0.0982 |
| 1250.00 | 0.2739 | 0.2848 | 0.2794 | 0.2724 |
| 2500.00 | 0.7521 | 0.7844 | 0.7683 | 0.7613 |
| 5000.00 | 1.9240 | 1.9110 | 1.9175 | 1.9106 |
| 10000.00 | 3.6510 | 3.6680 | 3.6595 | 3.6526 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 184.3 | 899.0 | 2654.9 | 187.9 | 893.6 | 2794.1 |
| Standard Deviation | 14.1 | 56.6 | 92.1 | 6.7 | 37.5 | 36.0 |
| Coefficient of Variation(%) | 7.6 | 6.3 | 3.5 | 3.6 | 4.2 | 1.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human alpha Fetoprotein into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 110% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 969.07-5411.95 | 100 | 2341.85 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of alpha Fetoprotein in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: alpha Fetoprotein
alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.
