Rat CRP/C-Reactive Protein enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 27.24 pg/ml (50 μl)；63.91 pg/ml (10 μl) |
| Standard Curve Range | 81.92~20000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0248 | 0.0257 | 0.0253 | |
| 81.92 | 0.0392 | 0.0371 | 0.0382 | 0.0232 |
| 204.80 | 0.0777 | 0.0792 | 0.0785 | 0.0635 |
| 512.00 | 0.1553 | 0.1399 | 0.1476 | 0.1326 |
| 1280.00 | 0.3510 | 0.3545 | 0.3528 | 0.3378 |
| 3200.00 | 0.9028 | 0.8789 | 0.8909 | 0.8759 |
| 8000.00 | 2.0410 | 2.1280 | 2.0845 | 2.0695 |
| 20000.00 | 3.6460 | 3.9210 | 3.7835 | 3.7685 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 486.6 | 2033.5 | 6246.1 | 483.8 | 2222.2 | 6448.2 |
| Standard Deviation | 25.5 | 121.9 | 330.3 | 15.4 | 112.6 | 123.97 |
| Coefficient of Variation(%) | 5.2 | 6.0 | 4.8 | 3.2 | 5.1 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat CRP into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 74% to 137% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 96.31-1708.35 | 100 | 398.28 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-8 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CRP/C-Reactive Protein
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
