Human CCL20/MIP-3 alpha enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 8.58 pg/ml (50 μl)；12.19 pg/ml (10 μl) |
| Standard Curve Range | 10.97~8000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0175 | 0.0195 | 0.0185 | |
| 10.97 | 0.0264 | 0.0238 | 0.0251 | 0.0066 |
| 32.92 | 0.0320 | 0.0311 | 0.0316 | 0.0131 |
| 98.77 | 0.0640 | 0.0629 | 0.0635 | 0.0450 |
| 296.30 | 0.2220 | 0.2240 | 0.2230 | 0.2045 |
| 888.89 | 0.9390 | 0.9138 | 0.9264 | 0.9079 |
| 2666.67 | 2.4702 | 2.4450 | 2.4576 | 2.4391 |
| 8000.00 | 3.9750 | 4.0220 | 3.9985 | 3.9800 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 78.1 | 395.8 | 1027.1 | 82.4 | 379.2 | 1127.4 |
| Standard Deviation | 2.4 | 14.8 | 30.9 | 4.2 | 24.1 | 74.6 |
| Coefficient of Variation(%) | 3.1 | 3.7 | 3.0 | 4.0 | 6.4 | 6.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL20 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 121% to 128% with an overall mean recovery of 115%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-67.43 | 20 | 39.61 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL20 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CCL20/MIP-3 alpha
CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.
