Rat IL-1β enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 17.11 pg/ml (50 μl) |
| Standard Curve Range | 10.97~8000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0472 | 0.0483 | 0.0478 | |
| 10.97 | 0.0572 | 0.0632 | 0.0602 | 0.0125 |
| 32.92 | 0.0723 | 0.0712 | 0.0718 | 0.0240 |
| 98.77 | 0.1191 | 0.1082 | 0.1137 | 0.0659 |
| 296.30 | 0.2382 | 0.2332 | 0.2357 | 0.1880 |
| 888.89 | 0.5772 | 0.5553 | 0.5663 | 0.5185 |
| 2666.67 | 1.3862 | 1.3711 | 1.3787 | 1.3309 |
| 8000.00 | 3.1091 | 3.0882 | 3.0987 | 3.0509 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 104.1 | 635.0 | 2135.6 | 113.2 | 656.1 | 2205.3 |
| Standard Deviation | 4.9 | 25.3 | 88.1 | 7.5 | 31.5 | 119.1 |
| Coefficient of Variation(%) | 4.7 | 4.0 | 4.1 | 6.6 | 4.8 | 5.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat IL-1 beta into health rat serum sample. The un-spiked serum was used as blank in these experiments.
The recovery ranged from 83% to 110% with an overall mean recovery of 92%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 27.9-613.9 | 63 | 155.1 |
Serum/Plasma – Thirty samples from apparently healthy rats were evaluated for the presence of IL-6 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-1β
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects.
IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
