Mouse IL-5 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.24 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0140 | 0.0130 | 0.0135 | |
| 6.86 | 0.0265 | 0.0280 | 0.0273 | 0.0138 |
| 20.58 | 0.0563 | 0.0497 | 0.0530 | 0.0395 |
| 61.73 | 0.1298 | 0.1234 | 0.1266 | 0.1131 |
| 185.19 | 0.3600 | 0.3211 | 0.3406 | 0.3271 |
| 555.56 | 1.0730 | 0.9880 | 1.0305 | 1.0170 |
| 1666.67 | 2.6440 | 2.3745 | 2.5093 | 2.4958 |
| 5000.00 | 4.0920 | 4.1950 | 4.1435 | 4.1300 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 83.1 | 612.8 | 1683.0 | 86.6 | 593.2 | 1495.4 |
| Standard Deviation | 4.7 | 23.9 | 69.5 | 4.7 | 31.0 | 77.8 |
| Coefficient of Variation(%) | 5.7 | 3.9 | 4.1 | 5.5 | 5.2 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-5 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 117% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.09-40.12 | 100 | 9.86 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL1/KC in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-5
IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
