Mouse IL-4 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.35 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0351 | 0.0332 | 0.0342 | |
| 2.74 | 0.0571 | 0.0641 | 0.0606 | 0.038 |
| 8.23 | 0.0947 | 0.0921 | 0.0934 | 0.0708 |
| 24.69 | 0.1691 | 0.1622 | 0.1657 | 0.1431 |
| 74.07 | 0.3641 | 0.3542 | 0.3592 | 0.3366 |
| 222.22 | 0.8632 | 0.8337 | 0.8485 | 0.8259 |
| 666.67 | 2.1632 | 2.1331 | 2.1482 | 2.1256 |
| 2000 | 3.9181 | 3.9331 | 3.9256 | 3.903 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 25.6 | 135.5 | 374.7 | 21.1 | 102.4 | 438.7 |
| Standard Deviation | 1.1 | 4.7 | 13.8 | 1.0 | 8.4 | 35.2 |
| Coefficient of Variation(%) | 4.5 | 3.5 | 3.7 | 4.9 | 8.2 | 8.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-4 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 120% with an overall mean recovery of 112%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-0.37 | 4.2 | 0.37 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-4 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-4
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.
