Mouse IL-22 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 11.2 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0691 | 0.0692 | 0.0692 | |
| 13.72 | 0.0762 | 0.0771 | 0.0767 | 0.0075 |
| 41.15 | 0.0931 | 0.0961 | 0.0946 | 0.0254 |
| 123.46 | 0.1505 | 0.1532 | 0.1519 | 0.0827 |
| 370.37 | 0.3031 | 0.3022 | 0.3027 | 0.2335 |
| 1111.11 | 0.7051 | 0.6984 | 0.7018 | 0.6326 |
| 3333.33 | 1.7461 | 1.7731 | 1.7596 | 1.6904 |
| 10000.00 | 3.6061 | 3.5883 | 3.5972 | 3.5280 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 145.2 | 826.5 | 3063.2 | 135.4 | 867.1 | 2965.3 |
| Standard Deviation | 8.1 | 46.4 | 8.1 | 7.2 | 49.7 | 93.6 |
| Coefficient of Variation(%) | 5.6 | 5.6 | 4.8 | 5.3 | 5.7 | 3.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-22 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 104% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-679.5 | 75 | 108.1 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-22 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-22
IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
