Mouse IL-17E enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 12.33 pg/ml (10 μl) |
| Standard Curve Range | 468.75~30000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0362 | 0.0324 | 0.0343 | |
| 468.75 | 0.0733 | 0.0788 | 0.0761 | 0.0540 |
| 937.50 | 0.1251 | 0.1218 | 0.1235 | 0.1014 |
| 1875.00 | 0.2239 | 0.2252 | 0.2246 | 0.2025 |
| 3750.00 | 0.4344 | 0.4357 | 0.4351 | 0.4130 |
| 7500.00 | 0.8770 | 0.8357 | 0.8564 | 0.8343 |
| 15000.00 | 1.8420 | 1.8410 | 1.8415 | 1.8195 |
| 30000.00 | 3.7110 | 3.8330 | 3.7720 | 3.7500 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 573.6 | 2641.9 | 7988.6 | 582.2 | 2756.5 | 8431.0 |
| Standard Deviation | 38.4 | 115.8 | 476.7 | 38.0 | 133.9 | 514,2 |
| Coefficient of Variation(%) | 6.7 | 4.4 | 6.0 | 6.5 | 4.9 | 6.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-17E/IL-25 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 118% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-427.60 | 75 | 199.53 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-17E/IL-25 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-17E
The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
