Mouse IL-12/IL-23 p40 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.50 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0405 | 0.0362 | 0.0384 | |
| 13.72 | 0.0520 | 0.0521 | 0.0521 | 0.0137 |
| 41.15 | 0.0770 | 0.0753 | 0.0762 | 0.0378 |
| 123.46 | 0.1401 | 0.1290 | 0.1346 | 0.0962 |
| 370.37 | 0.2752 | 0.2770 | 0.2761 | 0.2378 |
| 1111.11 | 0.6534 | 0.6611 | 0.6573 | 0.6189 |
| 3333.33 | 1.6875 | 1.6573 | 1.6724 | 1.6341 |
| 10000.00 | 3.3852 | 3.5716 | 3.4784 | 3.4401 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 129.9 | 875.6 | 2911.3 | 115.3 | 709.7 | 2588.1 |
| Standard Deviation | 6.1 | 48.8 | 113.2 | 8.7 | 39.1 | 150.6 |
| Coefficient of Variation(%) | 4.7 | 5.6 | 3.9 | 7.5 | 5.5 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-12/IL-23 p40 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 95% with an overall mean recovery of 87%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 3.09-102.65 | 91.7 | 42.16 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-12/IL-23 p40
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
