Mouse CXCL9/MIG enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.29 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0131 | 0.0121 | 0.0126 | |
| 6.86 | 0.0366 | 0.0386 | 0.0376 | 0.0250 |
| 20.58 | 0.0672 | 0.0673 | 0.0673 | 0.0547 |
| 61.73 | 0.1263 | 0.1294 | 0.1279 | 0.1153 |
| 185.19 | 0.3281 | 0.3382 | 0.3332 | 0.3206 |
| 555.56 | 0.9141 | 0.9123 | 0.9132 | 0.9006 |
| 1666.67 | 2.2132 | 2.2114 | 2.2123 | 2.1997 |
| 5000.00 | 4.0621 | 4.2362 | 4.1492 | 4.1366 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 85.9 | 497.9 | 1652.9 | 84.2 | 489.6 | 1546.5 |
| Standard Deviation | 5.8 | 28.0 | 102.9 | 4.2 | 25.7 | 52.5 |
| Coefficient of Variation(%) | 6.8 | 5.6 | 6.2 | 5.0 | 5.3 | 3.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL9/MIG into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 99% to 116% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.83-108.25 | 100 | 34.68 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL9/MIG in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CXCL9/MIG
CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.
