Mouse CXCL10/IP-10/CRG-2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 213.35 pg/ml (10 μl) |
| Standard Curve Range | 312.5~20000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0714 | 0.0721 | 0.0718 | |
| 625.00 | 0.1255 | 0.1243 | 0.1249 | 0.053 |
| 1250.00 | 0.3262 | 0.3452 | 0.3357 | 0.264 |
| 2500.00 | 0.7720 | 0.7891 | 0.7806 | 0.709 |
| 5000.00 | 1.7940 | 1.6820 | 1.7380 | 1.666 |
| 10000.00 | 2.8290 | 2.8190 | 2.8240 | 2.752 |
| 20000.00 | 4.1393 | 3.9812 | 4.0603 | 3.989 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 1023.2 | 3998.7 | 8588.8 | 1179.3 | 4202.1 | 8465.1 |
| Standard Deviation | 62.1 | 188.3 | 664.3 | 79.0 | 111.8 | 378.3 |
| Coefficient of Variation(%) | 6.1 | 4.7 | 7.7 | 6.7 | 2.7 | 4.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CXCL10 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 114% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-437.15 | 21 | 279.03 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL10 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CXCL10/IP-10/CRG-2
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
