Mouse CCL2/MCP-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.78 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0074 | 0.0068 | 0.0071 | |
| 6.86 | 0.0115 | 0.0114 | 0.0115 | 0.0044 |
| 20.58 | 0.0150 | 0.0166 | 0.0158 | 0.0087 |
| 61.73 | 0.0318 | 0.0326 | 0.0322 | 0.0251 |
| 185.19 | 0.0893 | 0.0986 | 0.0940 | 0.0869 |
| 555.56 | 0.2921 | 0.3009 | 0.2965 | 0.2894 |
| 1666.67 | 1.1280 | 1.1620 | 1.1450 | 1.1379 |
| 5000.00 | 3.5970 | 3.5747 | 3.5859 | 3.5788 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 122.5 | 532.2 | 1655.9 | 135.6 | 526.3 | 1598.4 |
| Standard Deviation | 9.1 | 23.5 | 68.1 | 8.4 | 24.1 | 60.7 |
| Coefficient of Variation(%) | 7.4 | 4.4 | 4.1 | 6.2 | 4.6 | 3.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CCL2 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 119% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 29.88-82.71 | 100 | 51.74 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CCL2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CCL2/MCP-1
CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
