Lentivirus HIV-1 p24 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.12 ng/ml (10 μl) |
| Standard Curve Range | 4.12~3000 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 1 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 75min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0072 | 0.0068 | 0.0070 | |
| 4.12 | 0.0220 | 0.0212 | 0.0216 | 0.0146 |
| 12.35 | 0.0453 | 0.0461 | 0.0457 | 0.0387 |
| 37.04 | 0.1085 | 0.1066 | 0.1076 | 0.1006 |
| 111.11 | 0.2794 | 0.2775 | 0.2785 | 0.2715 |
| 333.33 | 0.6978 | 0.6874 | 0.6926 | 0.6856 |
| 1000.00 | 1.7410 | 1.6832 | 1.7121 | 1.7051 |
| 3000.00 | 2.7224 | 2.6821 | 2.7023 | 2.6953 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 62.5 | 333.8 | 928.4 | 62.6 | 324.3 | 978.5 |
| Standard Deviation | 3.1 | 15.9 | 58.3 | 1.8 | 6.0 | 39.4 |
| Coefficient of Variation(%) | 4.9 | 4.8 | 6.3 | 3.9 | 1.9 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of HIV-1 p24. The recovery ranged from 95% to 106% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum |
Product Data Sheet
Background: HIV-1 p24
The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.
