Human YKL-40/CHI3L1 enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50 μl)；0.05 ng/ml (10 μl)； |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0057 | 0.0061 | |
| 0.14 | 0.0142 | 0.0134 | 0.0138 | 0.0078 |
| 0.41 | 0.0331 | 0.0298 | 0.0315 | 0.0254 |
| 1.23 | 0.0889 | 0.0861 | 0.0875 | 0.0815 |
| 3.70 | 0.2568 | 0.2291 | 0.2430 | 0.2369 |
| 11.11 | 0.7598 | 0.7190 | 0.7394 | 0.7334 |
| 33.33 | 1.8610 | 1.8180 | 1.8395 | 1.8335 |
| 100.00 | 3.3150 | 3.3390 | 3.3270 | 3.3210 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.8 | 9.7 | 32.4 | 1.8 | 9.2 | 31.1 |
| Standard Deviation | 0.0 | 0.3 | 1.4 | 0.1 | 0.4 | 1.8 |
| Coefficient of Variation(%) | 2.6 | 3.1 | 4.4 | 4.2 | 4.3 | 5.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human YKL-40/CHI3L1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 118% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 21.98-59.03 | 100 | 35.37 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of YKL-40/CHI3L1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: YKL-40/CHI3L1
Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
