Human VCAM-1 / CD106 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 7.07 pg/ml (50 μl) |
| Standard Curve Range | 41.15~30000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0090 | 0.0093 | 0.0092 | |
| 41.15 | 0.0130 | 0.0118 | 0.0124 | 0.0033 |
| 123.46 | 0.0190 | 0.0189 | 0.0190 | 0.0098 |
| 370.37 | 0.0495 | 0.0436 | 0.0466 | 0.0374 |
| 1111.11 | 0.1538 | 0.1445 | 0.1492 | 0.1400 |
| 3333.33 | 0.5925 | 0.5271 | 0.5598 | 0.5507 |
| 10000.00 | 1.9090 | 1.7580 | 1.8335 | 1.8244 |
| 30000.00 | 3.4470 | 3.4170 | 3.4320 | 3.4229 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 646.6 | 3231.2 | 10895.8 | 635.0 | 3119.9 | 10903.3 |
| Standard Deviation | 27.9 | 158.1 | 698.2 | 47.7 | 184.0 | 787.2 |
| Coefficient of Variation(%) | 4.3 | 4.9 | 6.4 | 7.5 | 5.9 | 7.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human VCAM-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 117% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 212.34-306.21 | 100 | 255.91 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ICAM-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: VCAM-1 / CD106
VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.
